rabbit anti ang 2  (Danaher Inc)

Journal: Molecular Medicine Reports

Article Title: Hypoxia in utero increases the risk of pulmonary hypertension in rat offspring and is associated with vasopressin type-2 receptor upregulation

doi: 10.3892/mmr.2020.11533

Figure Lengend Snippet: Schematic diagram of rat maternal hypoxia treatment and measurement methods in different offspring age groups. HIF-1α, hypoxia-inducible factor-1α; HIF-2α, hypoxia-inducible factor-2α; V1R, vasopressin type-1 receptor; V2R, vasopressin type-2 receptor; PLGF, placental growth factor; TGF-β, Transforming growth factor-β 1; Ang-2, angiopoietin-2; TNF-α, tumor necrosis factor-α; α-SMA, α-smooth muscle aorta.

Article Snippet: Following blocking at room overnight at 4°C in TBS-T (containing fat-free milk 5%), membranes were incubated with the following primary antibodies: Mouse anti-HIF-1α (1:500, cat. no. Ab113642; Abcam); rabbit anti-HIF-2α (1:1,000, cat. no. Ab109616; Abcam); rabbit anti-α-SMA (1:300, cat. no. Ab5694; Abcam); rabbit anti-Ang-2 (1:1,000, cat. no. Ab180820; Abcam); rabbit anti-TNF-α (1:1,000, cat. no. Ab6671; Abcam); rabbit anti-TGF-β (1:400, cat. no. Ab92486, Abcam); mouse anti-PlGF (1:800, cat. no. RM0010-8F09; Novus Biologicals, LLC); rabbit anti-V2R (1:500, cat. no. ab176488; Abcam); and rabbit anti-GAPDH (1:2,000, cat. no. 5174; Cell Signaling Technology, Inc.), overnight at 4°C.

Techniques:

Journal: Neuromolecular Medicine

Article Title: A Short Bout of Exercise Prior to Stroke Improves Functional Outcomes by Enhancing Angiogenesis

doi: 10.1007/s12017-019-08533-x

Figure Lengend Snippet: Immunohistochemical analyses of VEGFR2, Ang-2, vWF and CD34 in the peri-infarct area of the ischemic brain. a Representative merged images counterstained with DAPI (nuclear staining) (blue). Ang-2, VEGFR2, vWF and CD34 antibodies are represented by the green color (arrows). b Relative fluorescence intensity showed that Ang-2, VEGFR2, and CD34 were increased in the exercise groups. After exercise, vWF was decreased. No significant differences were observed between groups ( p > 0.05). Sham: ( n = 4); MCAO: ( n = 4); MCAO-30m: ( n = 4); MCAO-60m: ( n = 4). Bar = 50 µm. Error bars represent S.E.M

Article Snippet: Membranes were then incubated with the primary antibodies, anti-Angiopoietin 2 (Ang-2) rabbit polyclonal (1/500, Abcam Cat# ab153934), anti-VEGF Receptor 2 rabbit polyclonal (1/500, Novus Cat# NB100-530), and anti-VEGFA rabbit polyclonal (1/500, Abcam Cat# ab46154) at 4 °C for 14 h. After washing with PBST, the nitrocellulose membrane was incubated with donkey anti mouse IRDye800®CW secondary antibody (1/5000, LI-COR Biosciences Cat# 926-32212, RRID: AB_621847), or donkey anti-rabbit IRDye800®CW secondary antibody (1/5000, LI-COR Biosciences Cat# 926-32213) for 90 min at RT in the dark.

Techniques: Immunohistochemical staining, Staining, Fluorescence

Journal: Neuromolecular Medicine

Article Title: A Short Bout of Exercise Prior to Stroke Improves Functional Outcomes by Enhancing Angiogenesis

doi: 10.1007/s12017-019-08533-x

Figure Lengend Snippet: Western blot analysis of Ang2, VEGFR2, and VEGF in the ipsilateral ischemic striatum. Quantitative analysis showed a significant increase in Ang-2 and VEGFR2 expression in the ipsilateral ischemic striatum of the 30m and 60m exercise groups relative to the non-exercise group. The VEGF expression showed an increasing trend in the exercise group rats. Sham: ( n = 4); MCAO: ( n = 4); MCAO-30m: ( n = 4); MCAO-60m: ( n = 4). Data expressed as mean ± S.E.M. (* p < 0.05) were normalized to GAPDH levels. Error bars represent S.E.M

Article Snippet: Membranes were then incubated with the primary antibodies, anti-Angiopoietin 2 (Ang-2) rabbit polyclonal (1/500, Abcam Cat# ab153934), anti-VEGF Receptor 2 rabbit polyclonal (1/500, Novus Cat# NB100-530), and anti-VEGFA rabbit polyclonal (1/500, Abcam Cat# ab46154) at 4 °C for 14 h. After washing with PBST, the nitrocellulose membrane was incubated with donkey anti mouse IRDye800®CW secondary antibody (1/5000, LI-COR Biosciences Cat# 926-32212, RRID: AB_621847), or donkey anti-rabbit IRDye800®CW secondary antibody (1/5000, LI-COR Biosciences Cat# 926-32213) for 90 min at RT in the dark.

Techniques: Western Blot, Expressing